A recent clinical study published in the journal ofPolski Merkuriusz Lekarskirevealed that daily vitamin E supplementation (100 mg) positively influenced female hormonal indicators, particularly estradiol and luteinizing hormone (LH), potentially improving infertility treatment outcomes. Early-cycle supplementation showed greater increases in these hormones when compared with mid-cycle supplementation, indicating enhanced hormonal activity. The study enrolled 40 women aged between 18 and 35 years, all receiving infertility treatment. The participants were divided into 2 groups, where the Group A received vitamin E starting on the 4th day of their menstrual cycle, representing early-cycle supplementation and Group B received taking vitamin E during the middle of the cycle, around the expected time of ovulation. Blood samples were analyzed using an immunoassay system (Cobas E 411) to determine the hormone levels before and after supplementation. The results demonstrated a clear increase in serum vitamin E levels in both groups following supplementation, confirming that even short-term use significantly elevates antioxidant levels in the body. Also, the study found a significant rise in estradiol and LH concentrations after treatment when compared with baseline values. These two hormones play a critical role in ovulation and fertility regulation. Estradiol is essential for follicle development and endometrial preparation for implantation. Its elevation indicates improved ovarian activity. Luteinizing hormone, meanwhile, is responsible for triggering ovulation and its increase suggests a higher likelihood of successful egg release. Also, early-cycle supplementation (Group A) showed better hormonal responses than mid-cycle supplementation (Group B). Statistical analysis revealed that estradiol levels were significantly higher in Group A (P=0.04), while luteinizing hormone levels also showed a notable rise (P=0.02). This implies that initiating vitamin E earlier in the menstrual cycle enhances the body’s natural hormonal rhythm, potentially improving fertility outcomes. While follicle-stimulating hormone (FSH) and prolactin levels showed modest changes, the marked improvements in estradiol and LH are particularly significant. This research explained that vitamin E, being a potent antioxidant, may help stabilize cellular membranes and reduce oxidative stress in ovarian tissues, promoting better hormonal signaling and follicular health. Overall, the findings suggest that short-term vitamin E supplementation at 100 mg/day positively influences female reproductive hormones, especially when started early in the menstrual cycle. Further studies on larger populations are imperative to confirm the long-term benefits and potential clinical applications of this vitamin-based approach. Assi, M. A., Shamran, S. G., Mahdi, K. A., & Al-Fahham, A. A. (2025). Effect of short duration vitamin E supplementation on some reproductive hormones in infertile women. Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego, 53(5), 587–591.https://doi.org/10.36740/Merkur202505101