Researchers from a recent study have observed that NeoMTA Plus, ProRoot MTA and Biodentine materials extracts are not cytotoxic and do not induce apoptosis in the human dental pulp stem cells (hDPSCs).
The study is published in the Journal of Dental Sciences.
Dental pulp stem cells (DPSCs) play a crucial role in the tissue healing process through odontoblast like cell differentiation.
Calcium silicate cements, such as Mineral trioxide aggregate (MTA) and Biodentine, have a wide and successful range of various clinical dental applications. Tricalcium silicate cements are called dental biomaterials because of their physicochemical and biocompatibility.
NeoMTA Plus was developed with similar properties to MTA. It is stated that NeoMTA Plus does not cause discoloration in dental tissues, it has sufficient radiopacity and hydration with MTA and it shows the production of calcium hydroxide required to induce mineralized tissue formation.
Hence, Sinem Birant and associates from the Istanbul University-Cerrahpasa, Faculty of Dentistry, Department of Pedodontics, Istanbul Turkey conducted the present study to evaluate the biocompatibility and compare the potential invitro cytotoxic effects of NeoMTA Plus, ProRootMTA and Biodentine on human dental pulp stem cells (hDPSCs).
To assess the effects of NeoMTA Plus, ProRoot MTA and Biodentine extracts at 1st, 3rd and 7th d on hDPCs, cell populations was determined by flow cytometry using an Annexin V detection kit. The data were analyzed statistically using the Kruskal–Wallis test. A p < 0.05 was considered as statistically significant.
The results showed that all groups showed cell viability similar to that of the control group on 1st, 3rd and 7th d. Although Biodentine exhibited higher cell viability rates than the other material groups, no statistically significant differences were noted between the sampled days (p > 0.05).
Therefore, the authors concluded that "all materials extracts are not cytotoxic and do not induce apoptosis in the hDPSCs. These results suggest that all the tested materials can lead to positive outcomes when used as reparative biomaterials."